Construct: shRNA TRCN0000030954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_110475.2-2222s1c1
DNA Barcode:
GAGCACTATGAGGATCGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otud7b (229603)
Gene Description:
OTU domain containing 7B
Transcript:
RefSeq XM_110475.2 (NON-CURRENT)
Match location:
Position 2222 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCACTATGAGGATCGCAAT
Hairpin Sequence:
5'-CCGG-GAGCACTATGAGGATCGCAAT-CTCGAG-ATTGCGATCCTCATAGTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCACTATGAGGATCGCAATCTCGAGATTGCGATCCTCATAGTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCACTATGAGGATCGCAATCTCGAGATTGCGATCCTCATAGTGCTC-3'

Other clones with same target sequence:

(none)