Construct: shRNA TRCN0000030962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130880.1-1285s1c1
DNA Barcode:
CCTGTATATGAAAGCCTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otud7a (170711)
Gene Description:
OTU domain containing 7A
Transcript:
RefSeq NM_130880.1 (CURRENT)
Match location:
Position 1285 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000030962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTATATGAAAGCCTGGAA
Hairpin Sequence:
5'-CCGG-CCTGTATATGAAAGCCTGGAA-CTCGAG-TTCCAGGCTTTCATATACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTATATGAAAGCCTGGAACTCGAGTTCCAGGCTTTCATATACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTATATGAAAGCCTGGAACTCGAGTTCCAGGCTTTCATATACAGG-3'

Other clones with same target sequence:

(none)