Construct: shRNA TRCN0000031011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144851.2-1942s1c1
DNA Barcode:
GCCATATTTCCGAAAGCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Senp1 (223870)
Gene Description:
SUMO1/sentrin specific peptidase 1
Transcript:
RefSeq NM_144851.2 (NON-CURRENT)
Match location:
Position 1942 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATATTTCCGAAAGCGAAT
Hairpin Sequence:
5'-CCGG-GCCATATTTCCGAAAGCGAAT-CTCGAG-ATTCGCTTTCGGAAATATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATATTTCCGAAAGCGAATCTCGAGATTCGCTTTCGGAAATATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATATTTCCGAAAGCGAATCTCGAGATTCGCTTTCGGAAATATGGC-3'

Other clones with same target sequence:

  1. TRCN0000301208