Construct: shRNA TRCN0000031019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146003.2-2957s1c1
DNA Barcode:
CCCTTAATGAAGCTGCACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Senp6 (215351)
Gene Description:
SUMO/sentrin specific peptidase 6
Transcript:
RefSeq NM_146003.2 (CURRENT)
Match location:
Position 2957 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTAATGAAGCTGCACATT
Hairpin Sequence:
5'-CCGG-CCCTTAATGAAGCTGCACATT-CTCGAG-AATGTGCAGCTTCATTAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTAATGAAGCTGCACATTCTCGAGAATGTGCAGCTTCATTAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTAATGAAGCTGCACATTCTCGAGAATGTGCAGCTTCATTAAGGG-3'

Other clones with same target sequence:

(none)