Construct: shRNA TRCN0000031083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025909.1-1266s1c1
DNA Barcode:
GTGCCGATGTAAGAGCTAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oma1 (67013)
Gene Description:
OMA1 zinc metallopeptidase
Transcript:
RefSeq NM_025909.1 (NON-CURRENT)
Match location:
Position 1266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCGATGTAAGAGCTAGTT
Hairpin Sequence:
5'-CCGG-GTGCCGATGTAAGAGCTAGTT-CTCGAG-AACTAGCTCTTACATCGGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCGATGTAAGAGCTAGTTCTCGAGAACTAGCTCTTACATCGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCGATGTAAGAGCTAGTTCTCGAGAACTAGCTCTTACATCGGCAC-3'

Other clones with same target sequence:

  1. TRCN0000335429