Construct: shRNA TRCN0000031122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030711.2-1367s1c1
DNA Barcode:
GCTTGTATTCTGAATATGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erap1 (80898)
Gene Description:
endoplasmic reticulum aminopeptidase 1
Transcript:
RefSeq NM_030711.2 (NON-CURRENT)
Match location:
Position 1367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTATTCTGAATATGCTA
Hairpin Sequence:
5'-CCGG-GCTTGTATTCTGAATATGCTA-CTCGAG-TAGCATATTCAGAATACAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTATTCTGAATATGCTACTCGAGTAGCATATTCAGAATACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTATTCTGAATATGCTACTCGAGTAGCATATTCAGAATACAAGC-3'

Other clones with same target sequence:

  1. TRCN0000324289