Construct: shRNA TRCN0000031135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127387.4-1656s1c1
DNA Barcode:
GCAGGTGTTAAGACCCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010111I01Rik (72061)
Gene Description:
RIKEN cDNA 2010111I01 gene
Transcript:
RefSeq XM_127387.4 (NON-CURRENT)
Match location:
Position 1656 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTGTTAAGACCCAACAA
Hairpin Sequence:
5'-CCGG-GCAGGTGTTAAGACCCAACAA-CTCGAG-TTGTTGGGTCTTAACACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTGTTAAGACCCAACAACTCGAGTTGTTGGGTCTTAACACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTGTTAAGACCCAACAACTCGAGTTGTTGGGTCTTAACACCTGC-3'

Other clones with same target sequence:

(none)