Construct: shRNA TRCN0000031176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139293.1-550s1c1
DNA Barcode:
GCCATACTGAAGCACCTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ece2 (107522)
Gene Description:
endothelin converting enzyme 2
Transcript:
RefSeq NM_139293.1 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATACTGAAGCACCTACTT
Hairpin Sequence:
5'-CCGG-GCCATACTGAAGCACCTACTT-CTCGAG-AAGTAGGTGCTTCAGTATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATACTGAAGCACCTACTTCTCGAGAAGTAGGTGCTTCAGTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATACTGAAGCACCTACTTCTCGAGAAGTAGGTGCTTCAGTATGGC-3'

Other clones with same target sequence:

(none)