Construct: shRNA TRCN0000031202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013771.2-1883s1c1
DNA Barcode:
CCTGAGAATGACAGATGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Yme1l1 (27377)
Gene Description:
YME1-like 1 (S. cerevisiae)
Transcript:
RefSeq NM_013771.2 (NON-CURRENT)
Match location:
Position 1883 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAATGACAGATGGAAT
Hairpin Sequence:
5'-CCGG-CCTGAGAATGACAGATGGAAT-CTCGAG-ATTCCATCTGTCATTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAATGACAGATGGAATCTCGAGATTCCATCTGTCATTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAATGACAGATGGAATCTCGAGATTCCATCTGTCATTCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000326054