Construct: shRNA TRCN0000031214

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172823.1-1570s1c1
DNA Barcode:
GCTGGCATTTAAGTGGCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lmln (239833)
Gene Description:
leishmanolysin-like (metallopeptidase M8 family)
Transcript:
RefSeq NM_172823.1 (NON-CURRENT)
Match location:
Position 1570 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031214)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCATTTAAGTGGCGAAT
Hairpin Sequence:
5'-CCGG-GCTGGCATTTAAGTGGCGAAT-CTCGAG-ATTCGCCACTTAAATGCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCATTTAAGTGGCGAATCTCGAGATTCGCCACTTAAATGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCATTTAAGTGGCGAATCTCGAGATTCGCCACTTAAATGCCAGC-3'

Other clones with same target sequence:

(none)