Construct: shRNA TRCN0000031255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008605.1-55s1c1
DNA Barcode:
CCCATGAATGACAGTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mmp12 (17381)
Gene Description:
matrix metallopeptidase 12
Transcript:
RefSeq NM_008605.1 (NON-CURRENT)
Match location:
Position 55 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGAATGACAGTGAATTT
Hairpin Sequence:
5'-CCGG-CCCATGAATGACAGTGAATTT-CTCGAG-AAATTCACTGTCATTCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGAATGACAGTGAATTTCTCGAGAAATTCACTGTCATTCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGAATGACAGTGAATTTCTCGAGAAATTCACTGTCATTCATGGG-3'

Other clones with same target sequence:

(none)