Construct: shRNA TRCN0000031400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027918.1-1959s1c1
DNA Barcode:
GCTCTTTGGAAGAAATGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1300017J02Rik (71775)
Gene Description:
RIKEN cDNA 1300017J02 gene
Transcript:
RefSeq NM_027918.1 (NON-CURRENT)
Match location:
Position 1959 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTTGGAAGAAATGGATT
Hairpin Sequence:
5'-CCGG-GCTCTTTGGAAGAAATGGATT-CTCGAG-AATCCATTTCTTCCAAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTTGGAAGAAATGGATTCTCGAGAATCCATTTCTTCCAAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTTGGAAGAAATGGATTCTCGAGAATCCATTTCTTCCAAAGAGC-3'

Other clones with same target sequence:

(none)