Construct: shRNA TRCN0000031421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178630.2-595s1c1
DNA Barcode:
GCCTGGAAATAGAGCCTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agbl3 (76223)
Gene Description:
ATP/GTP binding protein-like 3
Transcript:
RefSeq NM_178630.2 (NON-CURRENT)
Match location:
Position 595 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGAAATAGAGCCTGTTT
Hairpin Sequence:
5'-CCGG-GCCTGGAAATAGAGCCTGTTT-CTCGAG-AAACAGGCTCTATTTCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGAAATAGAGCCTGTTTCTCGAGAAACAGGCTCTATTTCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGAAATAGAGCCTGTTTCTCGAGAAACAGGCTCTATTTCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000331899