Construct: shRNA TRCN0000031421
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_178630.2-595s1c1
- DNA Barcode:
- GCCTGGAAATAGAGCCTGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Agbl3 (76223)
- Gene Description:
- ATP/GTP binding protein-like 3
- Transcript:
- RefSeq NM_178630.2 (NON-CURRENT)
- Match location:
- Position 595 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000031421)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTGGAAATAGAGCCTGTTT
- Hairpin Sequence:
-
5'-CCGG-GCCTGGAAATAGAGCCTGTTT-CTCGAG-AAACAGGCTCTATTTCCAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTGGAAATAGAGCCTGTTTCTCGAGAAACAGGCTCTATTTCCAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTGGAAATAGAGCCTGTTTCTCGAGAAACAGGCTCTATTTCCAGGC-3'
Other clones with same target sequence:
-
TRCN0000331899
