Construct: shRNA TRCN0000031423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178630.2-1140s1c1
DNA Barcode:
CCATACACTTACTCCAACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agbl3 (76223)
Gene Description:
ATP/GTP binding protein-like 3
Transcript:
RefSeq NM_178630.2 (NON-CURRENT)
Match location:
Position 1140 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACACTTACTCCAACCTT
Hairpin Sequence:
5'-CCGG-CCATACACTTACTCCAACCTT-CTCGAG-AAGGTTGGAGTAAGTGTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACACTTACTCCAACCTTCTCGAGAAGGTTGGAGTAAGTGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACACTTACTCCAACCTTCTCGAGAAGGTTGGAGTAAGTGTATGG-3'

Other clones with same target sequence:

(none)