Construct: shRNA TRCN0000031459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199021.2-3300s1c1
DNA Barcode:
GCTTCTTTATTGAGCCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpp10 (269109)
Gene Description:
dipeptidylpeptidase 10
Transcript:
RefSeq NM_199021.2 (NON-CURRENT)
Match location:
Position 3300 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTTTATTGAGCCAAATA
Hairpin Sequence:
5'-CCGG-GCTTCTTTATTGAGCCAAATA-CTCGAG-TATTTGGCTCAATAAAGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTTTATTGAGCCAAATACTCGAGTATTTGGCTCAATAAAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTTTATTGAGCCAAATACTCGAGTATTTGGCTCAATAAAGAAGC-3'

Other clones with same target sequence:

(none)