Construct: shRNA TRCN0000031580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_134720.5-1221s1c1
DNA Barcode:
CGCTGCCATTATTACTGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamts15 (235130)
Gene Description:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15
Transcript:
RefSeq XM_134720.5 (NON-CURRENT)
Match location:
Position 1221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGCCATTATTACTGACTT
Hairpin Sequence:
5'-CCGG-CGCTGCCATTATTACTGACTT-CTCGAG-AAGTCAGTAATAATGGCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGCCATTATTACTGACTTCTCGAGAAGTCAGTAATAATGGCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGCCATTATTACTGACTTCTCGAGAAGTCAGTAATAATGGCAGCG-3'

Other clones with same target sequence:

(none)