Construct: shRNA TRCN0000031580
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_134720.5-1221s1c1
- DNA Barcode:
- CGCTGCCATTATTACTGACTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Adamts15 (235130)
- Gene Description:
- a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15
- Transcript:
- RefSeq XM_134720.5 (NON-CURRENT)
- Match location:
- Position 1221 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000031580)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCTGCCATTATTACTGACTT
- Hairpin Sequence:
-
5'-CCGG-CGCTGCCATTATTACTGACTT-CTCGAG-AAGTCAGTAATAATGGCAGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCTGCCATTATTACTGACTTCTCGAGAAGTCAGTAATAATGGCAGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCTGCCATTATTACTGACTTCTCGAGAAGTCAGTAATAATGGCAGCG-3'
Other clones with same target sequence:
(none)
