Construct: shRNA TRCN0000031615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172146.1-295s1c1
DNA Barcode:
GCCTAAATTCAGGGTGCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppat (231327)
Gene Description:
phosphoribosyl pyrophosphate amidotransferase
Transcript:
RefSeq NM_172146.1 (NON-CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAAATTCAGGGTGCATAA
Hairpin Sequence:
5'-CCGG-GCCTAAATTCAGGGTGCATAA-CTCGAG-TTATGCACCCTGAATTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAAATTCAGGGTGCATAACTCGAGTTATGCACCCTGAATTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAAATTCAGGGTGCATAACTCGAGTTATGCACCCTGAATTTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000288013