Construct: shRNA TRCN0000031625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172511.2-301s1c1
DNA Barcode:
CGCCTTTATAAGGTTTGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abhd10 (213012)
Gene Description:
abhydrolase domain containing 10
Transcript:
RefSeq NM_172511.2 (NON-CURRENT)
Match location:
Position 301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTTATAAGGTTTGACTA
Hairpin Sequence:
5'-CCGG-CGCCTTTATAAGGTTTGACTA-CTCGAG-TAGTCAAACCTTATAAAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTTATAAGGTTTGACTACTCGAGTAGTCAAACCTTATAAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTTATAAGGTTTGACTACTCGAGTAGTCAAACCTTATAAAGGCG-3'

Other clones with same target sequence:

(none)