Construct: shRNA TRCN0000031746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008456.1-515s1c1
DNA Barcode:
GCAGCATTACACCCGTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klk1b5 (16622)
Gene Description:
kallikrein 1-related peptidase b5
Transcript:
RefSeq NM_008456.1 (NON-CURRENT)
Match location:
Position 515 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATTACACCCGTCATAT
Hairpin Sequence:
5'-CCGG-GCAGCATTACACCCGTCATAT-CTCGAG-ATATGACGGGTGTAATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATTACACCCGTCATATCTCGAGATATGACGGGTGTAATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATTACACCCGTCATATCTCGAGATATGACGGGTGTAATGCTGC-3'

Other clones with same target sequence:

(none)