Construct: shRNA TRCN0000031761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011187.1-191s1c1
DNA Barcode:
GCAACTGAAGGGATGGTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmb7 (19177)
Gene Description:
proteasome (prosome, macropain) subunit, beta type 7
Transcript:
RefSeq NM_011187.1 (CURRENT)
Match location:
Position 191 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTGAAGGGATGGTTGTT
Hairpin Sequence:
5'-CCGG-GCAACTGAAGGGATGGTTGTT-CTCGAG-AACAACCATCCCTTCAGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTGAAGGGATGGTTGTTCTCGAGAACAACCATCCCTTCAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTGAAGGGATGGTTGTTCTCGAGAACAACCATCCCTTCAGTTGC-3'

Other clones with same target sequence:

(none)