Construct: shRNA TRCN0000031782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010168.1-1420s1c1
DNA Barcode:
CCTGTACCCTTCAGTGACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
F2 (14061)
Gene Description:
coagulation factor II
Transcript:
RefSeq NM_010168.1 (NON-CURRENT)
Match location:
Position 1420 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTACCCTTCAGTGACTAT
Hairpin Sequence:
5'-CCGG-CCTGTACCCTTCAGTGACTAT-CTCGAG-ATAGTCACTGAAGGGTACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTACCCTTCAGTGACTATCTCGAGATAGTCACTGAAGGGTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTACCCTTCAGTGACTATCTCGAGATAGTCACTGAAGGGTACAGG-3'

Other clones with same target sequence:

(none)