Construct: shRNA TRCN0000031826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025633.1-595s1c1
DNA Barcode:
GAAACCTTTCTGGTGGGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Metap1d (66559)
Gene Description:
methionyl aminopeptidase type 1D (mitochondrial)
Transcript:
RefSeq NM_025633.1 (NON-CURRENT)
Match location:
Position 595 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCTTTCTGGTGGGCAAT
Hairpin Sequence:
5'-CCGG-GAAACCTTTCTGGTGGGCAAT-CTCGAG-ATTGCCCACCAGAAAGGTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCTTTCTGGTGGGCAATCTCGAGATTGCCCACCAGAAAGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCTTTCTGGTGGGCAATCTCGAGATTGCCCACCAGAAAGGTTTC-3'

Other clones with same target sequence:

(none)