Construct: shRNA TRCN0000031864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009356.1-1120s1c1
DNA Barcode:
CAGGGATCTGTGATGGAGCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prss40 (21756)
Gene Description:
protease, serine 40
Transcript:
RefSeq NM_009356.1 (NON-CURRENT)
Match location:
Position 1120 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGATCTGTGATGGAGCCA
Hairpin Sequence:
5'-CCGG-CAGGGATCTGTGATGGAGCCA-CTCGAG-TGGCTCCATCACAGATCCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGATCTGTGATGGAGCCACTCGAGTGGCTCCATCACAGATCCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGATCTGTGATGGAGCCACTCGAGTGGCTCCATCACAGATCCCTG-3'

Other clones with same target sequence:

(none)