Construct: shRNA TRCN0000031870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007400.1-1724s1c1
DNA Barcode:
GCCAATGTGTACCTACATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam12 (11489)
Gene Description:
a disintegrin and metallopeptidase domain 12 (meltrin alpha)
Transcript:
RefSeq NM_007400.1 (NON-CURRENT)
Match location:
Position 1724 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGTGTACCTACATGAT
Hairpin Sequence:
5'-CCGG-GCCAATGTGTACCTACATGAT-CTCGAG-ATCATGTAGGTACACATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGTGTACCTACATGATCTCGAGATCATGTAGGTACACATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGTGTACCTACATGATCTCGAGATCATGTAGGTACACATTGGC-3'

Other clones with same target sequence:

(none)