Construct: shRNA TRCN0000031932
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011971.2-397s1c1
- DNA Barcode:
- GAAGACCTTCAAGCCCTTCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Psmb3 (26446)
- Gene Description:
- proteasome (prosome, macropain) subunit, beta type 3
- Transcript:
- RefSeq NM_011971.2 (NON-CURRENT)
- Match location:
- Position 397 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000031932)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGACCTTCAAGCCCTTCAT
- Hairpin Sequence:
-
5'-CCGG-GAAGACCTTCAAGCCCTTCAT-CTCGAG-ATGAAGGGCTTGAAGGTCTTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGACCTTCAAGCCCTTCATCTCGAGATGAAGGGCTTGAAGGTCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGACCTTCAAGCCCTTCATCTCGAGATGAAGGGCTTGAAGGTCTTC-3'
Other clones with same target sequence:
-
TRCN0000287733
