Construct: shRNA TRCN0000031932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011971.2-397s1c1
DNA Barcode:
GAAGACCTTCAAGCCCTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmb3 (26446)
Gene Description:
proteasome (prosome, macropain) subunit, beta type 3
Transcript:
RefSeq NM_011971.2 (NON-CURRENT)
Match location:
Position 397 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACCTTCAAGCCCTTCAT
Hairpin Sequence:
5'-CCGG-GAAGACCTTCAAGCCCTTCAT-CTCGAG-ATGAAGGGCTTGAAGGTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACCTTCAAGCCCTTCATCTCGAGATGAAGGGCTTGAAGGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACCTTCAAGCCCTTCATCTCGAGATGAAGGGCTTGAAGGTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000287733