Construct: shRNA TRCN0000031961

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177310.2-874s1c1
DNA Barcode:
GCCTTTCTTTACGCGAAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xpnpep3 (321003)
Gene Description:
X-prolyl aminopeptidase 3, mitochondrial
Transcript:
RefSeq NM_177310.2 (CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031961)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTCTTTACGCGAAGTTT
Hairpin Sequence:
5'-CCGG-GCCTTTCTTTACGCGAAGTTT-CTCGAG-AAACTTCGCGTAAAGAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTCTTTACGCGAAGTTTCTCGAGAAACTTCGCGTAAAGAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTCTTTACGCGAAGTTTCTCGAGAAACTTCGCGTAAAGAAAGGC-3'

Other clones with same target sequence:

(none)