Construct: shRNA TRCN0000031969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053243.1-309s1c1
DNA Barcode:
CCTGAACAATGACATCATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prss1 (114228)
Gene Description:
protease, serine 1 (trypsin 1)
Transcript:
RefSeq NM_053243.1 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000031969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAACAATGACATCATGTT
Hairpin Sequence:
5'-CCGG-CCTGAACAATGACATCATGTT-CTCGAG-AACATGATGTCATTGTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAACAATGACATCATGTTCTCGAGAACATGATGTCATTGTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAACAATGACATCATGTTCTCGAGAACATGATGTCATTGTTCAGG-3'

Other clones with same target sequence:

(none)