Construct: shRNA TRCN0000032049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007954.1-1685s1c1
DNA Barcode:
TGGGAGGCTCTGCCAGTCTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ces1c (13884)
Gene Description:
carboxylesterase 1C
Transcript:
RefSeq NM_007954.1 (NON-CURRENT)
Match location:
Position 1685 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAGGCTCTGCCAGTCTCA
Hairpin Sequence:
5'-CCGG-TGGGAGGCTCTGCCAGTCTCA-CTCGAG-TGAGACTGGCAGAGCCTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAGGCTCTGCCAGTCTCACTCGAGTGAGACTGGCAGAGCCTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAGGCTCTGCCAGTCTCACTCGAGTGAGACTGGCAGAGCCTCCCA-3'

Other clones with same target sequence:

(none)