Construct: shRNA TRCN0000032064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011782.1-543s1c1
DNA Barcode:
GCAGAGTATGAACGAATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamts5 (23794)
Gene Description:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Transcript:
RefSeq NM_011782.1 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTATGAACGAATTTAT
Hairpin Sequence:
5'-CCGG-GCAGAGTATGAACGAATTTAT-CTCGAG-ATAAATTCGTTCATACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTATGAACGAATTTATCTCGAGATAAATTCGTTCATACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTATGAACGAATTTATCTCGAGATAAATTCGTTCATACTCTGC-3'

Other clones with same target sequence:

(none)