Construct: shRNA TRCN0000032196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016770.2-264s1c1
DNA Barcode:
GCATGAATTGAAGGCTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Folh1 (53320)
Gene Description:
folate hydrolase 1
Transcript:
RefSeq NM_016770.2 (NON-CURRENT)
Match location:
Position 264 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGAATTGAAGGCTGAGAA
Hairpin Sequence:
5'-CCGG-GCATGAATTGAAGGCTGAGAA-CTCGAG-TTCTCAGCCTTCAATTCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGAATTGAAGGCTGAGAACTCGAGTTCTCAGCCTTCAATTCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGAATTGAAGGCTGAGAACTCGAGTTCTCAGCCTTCAATTCATGC-3'

Other clones with same target sequence:

(none)