Construct: shRNA TRCN0000032220

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011184.2-308s1c1
DNA Barcode:
GCTCGTTCATTAGCAGACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psma3 (19167)
Gene Description:
proteasome (prosome, macropain) subunit, alpha type 3
Transcript:
RefSeq NM_011184.2 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032220)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTTCATTAGCAGACATA
Hairpin Sequence:
5'-CCGG-GCTCGTTCATTAGCAGACATA-CTCGAG-TATGTCTGCTAATGAACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTTCATTAGCAGACATACTCGAGTATGTCTGCTAATGAACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTTCATTAGCAGACATACTCGAGTATGTCTGCTAATGAACGAGC-3'

Other clones with same target sequence:

  1. TRCN0000332756