Construct: shRNA TRCN0000032249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357002.1-340s1c1
DNA Barcode:
GCGACGCTAAAGCAGAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psma8 (73677)
Gene Description:
proteasome (prosome, macropain) subunit, alpha type, 8
Transcript:
RefSeq XM_357002.1 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACGCTAAAGCAGAAATAT
Hairpin Sequence:
5'-CCGG-GCGACGCTAAAGCAGAAATAT-CTCGAG-ATATTTCTGCTTTAGCGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACGCTAAAGCAGAAATATCTCGAGATATTTCTGCTTTAGCGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACGCTAAAGCAGAAATATCTCGAGATATTTCTGCTTTAGCGTCGC-3'

Other clones with same target sequence:

(none)