Construct: shRNA TRCN0000032260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024465.1-495s1c1
DNA Barcode:
GCCCTTTCATCTCGGTAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abhd12 (76192)
Gene Description:
abhydrolase domain containing 12
Transcript:
RefSeq NM_024465.1 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTTCATCTCGGTAGAAA
Hairpin Sequence:
5'-CCGG-GCCCTTTCATCTCGGTAGAAA-CTCGAG-TTTCTACCGAGATGAAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTTCATCTCGGTAGAAACTCGAGTTTCTACCGAGATGAAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTTCATCTCGGTAGAAACTCGAGTTTCTACCGAGATGAAAGGGC-3'

Other clones with same target sequence:

(none)