Construct: shRNA TRCN0000032270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008572.1-680s1c1
DNA Barcode:
CCTCGGGTATTCACCAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcpt8 (17231)
Gene Description:
mast cell protease 8
Transcript:
RefSeq NM_008572.1 (CURRENT)
Match location:
Position 680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGGGTATTCACCAGAATT
Hairpin Sequence:
5'-CCGG-CCTCGGGTATTCACCAGAATT-CTCGAG-AATTCTGGTGAATACCCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGGGTATTCACCAGAATTCTCGAGAATTCTGGTGAATACCCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGGGTATTCACCAGAATTCTCGAGAATTCTGGTGAATACCCGAGG-3'

Other clones with same target sequence:

(none)