Construct: shRNA TRCN0000032279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_134151.3-1022s1c1
DNA Barcode:
GCCAGTGTGAACGGAAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aga (11593)
Gene Description:
aspartylglucosaminidase
Transcript:
RefSeq XM_134151.3 (NON-CURRENT)
Match location:
Position 1022 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTGTGAACGGAAGTTAT
Hairpin Sequence:
5'-CCGG-GCCAGTGTGAACGGAAGTTAT-CTCGAG-ATAACTTCCGTTCACACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTGTGAACGGAAGTTATCTCGAGATAACTTCCGTTCACACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTGTGAACGGAAGTTATCTCGAGATAACTTCCGTTCACACTGGC-3'

Other clones with same target sequence:

(none)