Construct: shRNA TRCN0000032400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358328.1-336s1c1
DNA Barcode:
CCAAAGCCAAAGAGAGTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ermp1 (226090)
Gene Description:
endoplasmic reticulum metallopeptidase 1
Transcript:
RefSeq XM_358328.1 (NON-CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCCAAAGAGAGTGTTT
Hairpin Sequence:
5'-CCGG-CCAAAGCCAAAGAGAGTGTTT-CTCGAG-AAACACTCTCTTTGGCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCCAAAGAGAGTGTTTCTCGAGAAACACTCTCTTTGGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCCAAAGAGAGTGTTTCTCGAGAAACACTCTCTTTGGCTTTGG-3'

Other clones with same target sequence:

(none)