Construct: shRNA TRCN0000032408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008906.2-1149s1c1
DNA Barcode:
CCAAAGCATGAACTCCCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctsa (19025)
Gene Description:
cathepsin A
Transcript:
RefSeq NM_008906.2 (NON-CURRENT)
Match location:
Position 1149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCATGAACTCCCAGTA
Hairpin Sequence:
5'-CCGG-CCAAAGCATGAACTCCCAGTA-CTCGAG-TACTGGGAGTTCATGCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCATGAACTCCCAGTACTCGAGTACTGGGAGTTCATGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCATGAACTCCCAGTACTCGAGTACTGGGAGTTCATGCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000327337