Construct: shRNA TRCN0000032417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011176.3-2246s1c1
DNA Barcode:
GCTGAAGCTCAAACGTATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St14 (19143)
Gene Description:
suppression of tumorigenicity 14 (colon carcinoma)
Transcript:
RefSeq NM_011176.3 (NON-CURRENT)
Match location:
Position 2246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGCTCAAACGTATCAT
Hairpin Sequence:
5'-CCGG-GCTGAAGCTCAAACGTATCAT-CTCGAG-ATGATACGTTTGAGCTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGCTCAAACGTATCATCTCGAGATGATACGTTTGAGCTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGCTCAAACGTATCATCTCGAGATGATACGTTTGAGCTTCAGC-3'

Other clones with same target sequence:

(none)