Construct: shRNA TRCN0000032434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132566.1-661s1c1
DNA Barcode:
GCGAGTCTTATGCAGGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpvl (71287)
Gene Description:
carboxypeptidase, vitellogenic-like
Transcript:
RefSeq XM_132566.1 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGTCTTATGCAGGGAAAT
Hairpin Sequence:
5'-CCGG-GCGAGTCTTATGCAGGGAAAT-CTCGAG-ATTTCCCTGCATAAGACTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGTCTTATGCAGGGAAATCTCGAGATTTCCCTGCATAAGACTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGTCTTATGCAGGGAAATCTCGAGATTTCCCTGCATAAGACTCGC-3'

Other clones with same target sequence:

(none)