Construct: shRNA TRCN0000032449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009619.2-2236s1c1
DNA Barcode:
GCAGAATATCTCAAAGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam3 (11497)
Gene Description:
a disintegrin and metallopeptidase domain 3 (cyritestin)
Transcript:
RefSeq NM_009619.2 (NON-CURRENT)
Match location:
Position 2236 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATATCTCAAAGGCATT
Hairpin Sequence:
5'-CCGG-GCAGAATATCTCAAAGGCATT-CTCGAG-AATGCCTTTGAGATATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATATCTCAAAGGCATTCTCGAGAATGCCTTTGAGATATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATATCTCAAAGGCATTCTCGAGAATGCCTTTGAGATATTCTGC-3'

Other clones with same target sequence:

(none)