Construct: shRNA TRCN0000032551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_131981.5-5934s1c1
DNA Barcode:
GCAGTTCACTAAGGATCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cad (69719)
Gene Description:
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Transcript:
RefSeq XM_131981.5 (NON-CURRENT)
Match location:
Position 5934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTCACTAAGGATCAGAT
Hairpin Sequence:
5'-CCGG-GCAGTTCACTAAGGATCAGAT-CTCGAG-ATCTGATCCTTAGTGAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTCACTAAGGATCAGATCTCGAGATCTGATCCTTAGTGAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTCACTAAGGATCAGATCTCGAGATCTGATCCTTAGTGAACTGC-3'

Other clones with same target sequence:

(none)