Construct: shRNA TRCN0000032566

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030709.1-989s1c1
DNA Barcode:
GCCCAGAACCATGACTATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmprss5 (80893)
Gene Description:
transmembrane protease, serine 5 (spinesin)
Transcript:
RefSeq NM_030709.1 (NON-CURRENT)
Match location:
Position 989 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032566)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAACCATGACTATGAT
Hairpin Sequence:
5'-CCGG-GCCCAGAACCATGACTATGAT-CTCGAG-ATCATAGTCATGGTTCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAACCATGACTATGATCTCGAGATCATAGTCATGGTTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAACCATGACTATGATCTCGAGATCATAGTCATGGTTCTGGGC-3'

Other clones with same target sequence:

(none)