Construct: shRNA TRCN0000032634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139228.2-721s1c1
DNA Barcode:
GCGCTATGTGACGTACATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhbdl3 (246104)
Gene Description:
rhomboid, veinlet-like 3 (Drosophila)
Transcript:
RefSeq NM_139228.2 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTATGTGACGTACATCTT
Hairpin Sequence:
5'-CCGG-GCGCTATGTGACGTACATCTT-CTCGAG-AAGATGTACGTCACATAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTATGTGACGTACATCTTCTCGAGAAGATGTACGTCACATAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTATGTGACGTACATCTTCTCGAGAAGATGTACGTCACATAGCGC-3'

Other clones with same target sequence:

(none)