Construct: shRNA TRCN0000032783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_131437.4-2538s1c1
DNA Barcode:
CCTGGAGATTGATGCAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pappa (18491)
Gene Description:
pregnancy-associated plasma protein A
Transcript:
RefSeq XM_131437.4 (NON-CURRENT)
Match location:
Position 2538 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGATTGATGCAGCAAT
Hairpin Sequence:
5'-CCGG-CCTGGAGATTGATGCAGCAAT-CTCGAG-ATTGCTGCATCAATCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGATTGATGCAGCAATCTCGAGATTGCTGCATCAATCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGATTGATGCAGCAATCTCGAGATTGCTGCATCAATCTCCAGG-3'

Other clones with same target sequence:

(none)