Construct: shRNA TRCN0000032850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009418.1-2522s1c1
DNA Barcode:
GCCAAATAATCGCCAGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpp2 (22019)
Gene Description:
tripeptidyl peptidase II
Transcript:
RefSeq NM_009418.1 (NON-CURRENT)
Match location:
Position 2522 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAATAATCGCCAGCTTTA
Hairpin Sequence:
5'-CCGG-GCCAAATAATCGCCAGCTTTA-CTCGAG-TAAAGCTGGCGATTATTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAATAATCGCCAGCTTTACTCGAGTAAAGCTGGCGATTATTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAATAATCGCCAGCTTTACTCGAGTAAAGCTGGCGATTATTTGGC-3'

Other clones with same target sequence:

(none)