Construct: shRNA TRCN0000032888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011046.1-564s1c1
DNA Barcode:
GCCAGATCTTCGGTGACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Furin (18550)
Gene Description:
furin (paired basic amino acid cleaving enzyme)
Transcript:
RefSeq NM_011046.1 (NON-CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000032888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGATCTTCGGTGACTATT
Hairpin Sequence:
5'-CCGG-GCCAGATCTTCGGTGACTATT-CTCGAG-AATAGTCACCGAAGATCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGATCTTCGGTGACTATTCTCGAGAATAGTCACCGAAGATCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGATCTTCGGTGACTATTCTCGAGAATAGTCACCGAAGATCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000302220