Construct: shRNA TRCN0000033283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002649.2-940s1c1
DNA Barcode:
CCTGTGGAAGAAGATTGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3CG (5294)
Gene Description:
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Transcript:
RefSeq NM_002649.2 (NON-CURRENT)
Match location:
Position 940 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGAAGAAGATTGCCAA
Hairpin Sequence:
5'-CCGG-CCTGTGGAAGAAGATTGCCAA-CTCGAG-TTGGCAATCTTCTTCCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGAAGAAGATTGCCAACTCGAGTTGGCAATCTTCTTCCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGAAGAAGATTGCCAACTCGAGTTGGCAATCTTCTTCCACAGG-3'

Other clones with same target sequence:

(none)