Construct: shRNA TRCN0000033293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003629.2-1258s1c1
DNA Barcode:
GAGAAGAGTAAAGAGTATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3R3 (8503)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 3
Transcript:
RefSeq NM_003629.2 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGAGTAAAGAGTATGAT
Hairpin Sequence:
5'-CCGG-GAGAAGAGTAAAGAGTATGAT-CTCGAG-ATCATACTCTTTACTCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGAGTAAAGAGTATGATCTCGAGATCATACTCTTTACTCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGAGTAAAGAGTATGATCTCGAGATCATACTCTTTACTCTTCTC-3'

Other clones with same target sequence:

(none)