Construct: shRNA TRCN0000033303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370995.2-405s1c1
DNA Barcode:
AGACAGCCTGAACCACCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNAI3 (333929)
Gene Description:
snail family transcriptional repressor 3
Transcript:
RefSeq XM_370995.2 (NON-CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACAGCCTGAACCACCTCAA
Hairpin Sequence:
5'-CCGG-AGACAGCCTGAACCACCTCAA-CTCGAG-TTGAGGTGGTTCAGGCTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACAGCCTGAACCACCTCAACTCGAGTTGAGGTGGTTCAGGCTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACAGCCTGAACCACCTCAACTCGAGTTGAGGTGGTTCAGGCTGTCT-3'

Other clones with same target sequence:

(none)