Construct: shRNA TRCN0000033321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_050846.6-477s1c1
DNA Barcode:
CCGCAATAAGTATGGACTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IHH (3549)
Gene Description:
Indian hedgehog signaling molecule
Transcript:
RefSeq XM_050846.6 (NON-CURRENT)
Match location:
Position 477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAATAAGTATGGACTGCT
Hairpin Sequence:
5'-CCGG-CCGCAATAAGTATGGACTGCT-CTCGAG-AGCAGTCCATACTTATTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAATAAGTATGGACTGCTCTCGAGAGCAGTCCATACTTATTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAATAAGTATGGACTGCTCTCGAGAGCAGTCCATACTTATTGCGG-3'

Other clones with same target sequence:

(none)